[PDF] The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers | Semantic Scholar (2024)

Skip to search formSkip to main contentSkip to account menu

Semantic ScholarSemantic Scholar's Logo
@article{Emre2010TheRI, title={The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers}, author={N. C. Tolga Emre and Jason G. Vidal and Jeanne Elia and Eric D. O'Connor and Rosanto I. Paramban and Michael P. Hefferan and Roman Navarro and Danielle S. Goldberg and Nissi Varki and Martin Marsala and Christian T. Carson}, journal={PLoS ONE}, year={2010}, volume={5}, url={https://api.semanticscholar.org/CorpusID:18902463}}
  • N. Emre, J. Vidal, C. Carson
  • Published in PLoS ONE 13 August 2010
  • Biology, Medicine

The application of Y-27632 to hESCs after cell sorting improves cell recovery with no observed effect on pluripotency, and enables the consistent recovery of hESC by FACS using multiple surface markers.

69 Citations

Highly Influential Citations

1

Background Citations

15

Methods Citations

8

Results Citations

3

Figures from this paper

  • figure 1
  • figure 2
  • figure 3
  • figure 4
  • figure 5
  • figure 6
  • figure 7

69 Citations

Post-Passage rock inhibition induces cytoskeletal aberrations and apoptosis in Human embryonic stem cells.
    Lijie GaoSuman C. Nath D. Rancourt

    Medicine, Biology

    Stem cell research

  • 2019
  • 15
  • PDF
Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells.
    H. Kurosawa

    Biology, Medicine

    Journal of bioscience and bioengineering

  • 2012
  • 72
ROCK inhibitor primes human induced pluripotent stem cells to selectively differentiate towards mesendodermal lineage via epithelial-mesenchymal transition-like modulation.
    Maricela MaldonadoR. J. LuuMichael E P RamosJin Nam

    Medicine, Biology

    Stem cell research

  • 2016
  • 45
  • PDF
Rho-Associated Protein Kinase Inhibitor and Hypoxia Synergistically Enhance the Self-Renewal, Survival Rate, and Proliferation of Human Stem Cells
    Sarah F AlsobaieTamador AlsobaieSakis Mantalaris

    Medicine, Biology

  • 2022

This study provides the first evidence of the synergistic effect of ROCK inhibition and hypoxia on expanding viable pluripotent single-cell suspensions of human IPSCs and embryonic stem cells.

The ROCK Inhibitor Y-26732 Enhances the Survival and Proliferation of Human Embryonic Stem Cell-Derived Neural Progenitor Cells upon Dissociation
    R. RungsiwiwutChirawattana Manolertthewan K. Pruksananonda

    Biology, Medicine

    Cells Tissues Organs

  • 2013

The findings provide the optimal time window for ROCK treatment of hNPC dissociation in respect to apoptosis and cell proliferation and both supplementation conditions with Y-26732 enhanced the proliferation of dissociated hN PCs.

  • 12
Human embryonic and induced pluripotent stem cells maintain phenotype but alter their metabolism after exposure to ROCK inhibitor
    S. VernardisK. TerzoudisN. PanoskaltsisA. Mantalaris

    Biology, Medicine

    Scientific Reports

  • 2017

The usefulness of metabolomics in providing accurate and sensitive information on cellular physiological status, which could lead to the development of robust and optimal stem cell bioprocesses, is highlighted.

  • 45
  • PDF
Rho-Associated Protein Kinase Inhibitor and Hypoxia Synergistically Enhance the Self-Renewal, Survival Rate, and Proliferation of Human Stem Cells
    Sarah F AlsobaieTamador AlsobaieS. Mantalaris

    Medicine, Biology

    Stem cells and cloning : advances and…

  • 2022

The beneficial effect of the interaction between hypoxia and ROCK inhibition in regulating cell proliferation, pluripotency, and single-cell survival of pluripotent cells is shown.

ROCK inhibitor Y-27632 maintains the proliferation of confluent human mesenchymal stem cells.
    Kouhei NakamuraA. YoshimuraT. KanekoKayo SatoY. Hara

    Medicine

    Journal of periodontal research

  • 2014

Y-27632 both prevented the MSC sheets from detaching and maintained the multilayered proliferation of confluent MSCs by MFM, and then cells in the sheets had differentiation potency.

  • 24
  • PDF
Improving viability and transfection efficiency with human umbilical cord wharton's jelly cells through use of a ROCK inhibitor.
    Adam J. MellottMegan E. GodseyHeather E. ShinogleDavid S. MooreM. Laird ForrestM. Detamore

    Engineering, Medicine

    Cellular reprogramming

  • 2014

It is shown that using Y-27632-RI in concert with nucleofection could greatly enhance the utility of differentiating and reprogramming hUCMSCs for tissue engineering applications.

  • 15
  • PDF
ROCK inhibitor Y-27632 increases the cloning efficiency of limbal stem/progenitor cells by improving their adherence and ROS-scavenging capacity.
    Qingjun ZhouH. DuanYao WangMingli QuLingling YangLixin Xie

    Biology, Medicine

    Tissue engineering. Part C, Methods

  • 2013

Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 increased the cloning efficiency of rabbit limbal stem/progenitor cells by improving their adherence and ROS scavenging capacity.

  • 28
  • PDF

...

...

45 References

The ROCK inhibitor Y-27632 enhances the survival rate of human embryonic stem cells following cryopreservation.
    Xiangyun LiG. MengR. KrawetzShiying LiuD. Rancourt

    Medicine, Biology

    Stem cells and development

  • 2008

It is concluded that conventional slow freezing with Y-27632 treatment is efficient and convenient for the cryopreservation of hES cells.

  • 114
The ROCK Inhibitor Y-27632 Negatively Affects the Expansion/Survival of Both Fresh and Cryopreserved Cord Blood-Derived CD34+ Hematopoietic Progenitor Cells
    C. BuenoR. MontesP. Menéndez

    Biology, Medicine

    Stem Cell Reviews and Reports

  • 2010

This study identifies for the first time a detrimental effect of Y-27632 on the expansion and survival of both fresh and cryopreserved CB-derived CD34+ HSPCs, suggesting that Y- 27632 may have a differential impact on distinct lineage/tissue-specific stem cells.

  • 48
  • PDF
Presence of a ROCK Inhibitor in Extracellular Matrix Supports More Undifferentiated Growth of Feeder-Free Human Embryonic and Induced Pluripotent Stem Cells upon Passaging
    Mohammad PakzadM. TotonchiA. TaeiA. SeifinejadSeyedeh-Nafiseh HassaniH. Baharvand

    Biology, Medicine

    Stem Cell Reviews and Reports

  • 2009

Results show that addition of ROCKi in the extracellular matrix can increase the plating efficiency of hESCs and hiPSCs during passaging of clusters due not only to an anti-apoptotic effect, but also to an increase in the ECM-cells interaction.

  • 93
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
    Kiichi WatanabeMorio Ueno Y. Sasai

    Biology, Medicine

    Nature Biotechnology

  • 2007

Application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency and facilitates subcloning after gene transfer, and enables SFEB-cultured hES Cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors.

  • 2,001
  • PDF
ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells
    David ClaassenM. DeslerA. Rizzino

    Medicine, Biology

    Molecular reproduction and development

  • 2009

It is demonstrated that Y‐27632, a specific inhibitor of Rho kinase (ROCK) activity, significantly enhances recovery of hES cells from cryopreserved stocks when cultured with or without a growth inactivated feeder layer, and argues that this tool is a useful tool in overcoming obstacles to studies involving the cultivation of both h ES cells and human iPS cells.

  • 165
  • PDF
Effect of Rho-associated kinase (ROCK) inhibitor Y-27632 on the post-thaw viability of cryopreserved human bone marrow-derived mesenchymal stem cells.
    B. Heng

    Medicine

  • 2009
  • 73
Rho-associated kinase inhibitor Y-27632 promotes survival of cynomolgus monkey embryonic stem cells
    Toshiyuki TakeharaTakeshi Teramura Y. Hosoi

    Biology, Medicine

    Molecular human reproduction

  • 2008

The present result suggests that the chemical was effective for improving the culture system of primate ES cells, but also the similarity between cyES and hES cells regarding the reactions to the chemical, which might be further evidence that cyES cells are superior models for h ES cells.

Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor.
    Raquel Martín-IbañezRaquel Martín-Ibañez O. Hovatta

    Biology, Medicine

    Human reproduction

  • 2008

This novel method for the cryopreservation of dissociated hESCs may reduce the time required to amplify frozen stocks, and facilitate not only the storage of large numbers of hESC but also the widespread use of these cells in regenerative medicine.

  • 119
  • PDF
ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.
    Xiangyun LiR. KrawetzShiying LiuG. MengD. Rancourt

    Medicine, Biology

    Human reproduction

  • 2009

A novel method to increase freeze-thaw survival and proliferation rate of single hES cells in serum-free and feeder-free culture conditions and it is believed this method will be ideal for current and future hES cell applications that are targeted towards a therapeutic end-point.

  • 172
  • PDF
Inhibition of ROCK improves survival of human embryonic stem cell–derived cardiomyocytes after dissociation
    S. BraamRoy NauwD. Ward-van OostwaardC. MummeryR. Passier

    Biology, Medicine

    Annals of the New York Academy of Sciences

  • 2010

In conclusion, inhibition of the Rho‐associated kinase (ROCK) by Y‐27632 improved survival of dissociated hESC‐derived differentiated cells and facilitated development of applications of hESCs‐derived cardiomyocytes in multiple research areas.

  • 35

...

...

Related Papers

Showing 1 through 3 of 0 Related Papers

    [PDF] The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers | Semantic Scholar (2024)
    Top Articles
    Latest Posts
    Recommended Articles
    Article information

    Author: Merrill Bechtelar CPA

    Last Updated:

    Views: 5616

    Rating: 5 / 5 (50 voted)

    Reviews: 89% of readers found this page helpful

    Author information

    Name: Merrill Bechtelar CPA

    Birthday: 1996-05-19

    Address: Apt. 114 873 White Lodge, Libbyfurt, CA 93006

    Phone: +5983010455207

    Job: Legacy Representative

    Hobby: Blacksmithing, Urban exploration, Sudoku, Slacklining, Creative writing, Community, Letterboxing

    Introduction: My name is Merrill Bechtelar CPA, I am a clean, agreeable, glorious, magnificent, witty, enchanting, comfortable person who loves writing and wants to share my knowledge and understanding with you.