Skip to search formSkip to main contentSkip to account menu
DOI:10.1371/journal.pone.0012148 - Corpus ID: 18902463
@article{Emre2010TheRI, title={The ROCK Inhibitor Y-27632 Improves Recovery of Human Embryonic Stem Cells after Fluorescence-Activated Cell Sorting with Multiple Cell Surface Markers}, author={N. C. Tolga Emre and Jason G. Vidal and Jeanne Elia and Eric D. O'Connor and Rosanto I. Paramban and Michael P. Hefferan and Roman Navarro and Danielle S. Goldberg and Nissi Varki and Martin Marsala and Christian T. Carson}, journal={PLoS ONE}, year={2010}, volume={5}, url={https://api.semanticscholar.org/CorpusID:18902463}}
- N. Emre, J. Vidal, C. Carson
- Published in PLoS ONE 13 August 2010
- Biology, Medicine
The application of Y-27632 to hESCs after cell sorting improves cell recovery with no observed effect on pluripotency, and enables the consistent recovery of hESC by FACS using multiple surface markers.
69 Citations
1
15
8
3
Figures from this paper
- figure 1
- figure 2
- figure 3
- figure 4
- figure 5
- figure 6
- figure 7
69 Citations
- Lijie GaoSuman C. Nath D. Rancourt
- 2019
Medicine, Biology
Stem cell research
- 15
- PDF
- H. Kurosawa
- 2012
Biology, Medicine
Journal of bioscience and bioengineering
- 72
- Maricela MaldonadoR. J. LuuMichael E P RamosJin Nam
- 2016
Medicine, Biology
Stem cell research
- 45
- PDF
- Sarah F AlsobaieTamador AlsobaieSakis Mantalaris
- 2022
Medicine, Biology
This study provides the first evidence of the synergistic effect of ROCK inhibition and hypoxia on expanding viable pluripotent single-cell suspensions of human IPSCs and embryonic stem cells.
- R. RungsiwiwutChirawattana Manolertthewan K. Pruksananonda
- 2013
Biology, Medicine
Cells Tissues Organs
The findings provide the optimal time window for ROCK treatment of hNPC dissociation in respect to apoptosis and cell proliferation and both supplementation conditions with Y-26732 enhanced the proliferation of dissociated hN PCs.
- 12
- S. VernardisK. TerzoudisN. PanoskaltsisA. Mantalaris
- 2017
Biology, Medicine
Scientific Reports
The usefulness of metabolomics in providing accurate and sensitive information on cellular physiological status, which could lead to the development of robust and optimal stem cell bioprocesses, is highlighted.
- 45
- PDF
- Sarah F AlsobaieTamador AlsobaieS. Mantalaris
- 2022
Medicine, Biology
Stem cells and cloning : advances and…
The beneficial effect of the interaction between hypoxia and ROCK inhibition in regulating cell proliferation, pluripotency, and single-cell survival of pluripotent cells is shown.
- Kouhei NakamuraA. YoshimuraT. KanekoKayo SatoY. Hara
- 2014
Medicine
Journal of periodontal research
Y-27632 both prevented the MSC sheets from detaching and maintained the multilayered proliferation of confluent MSCs by MFM, and then cells in the sheets had differentiation potency.
- 24
- PDF
- Adam J. MellottMegan E. GodseyHeather E. ShinogleDavid S. MooreM. Laird ForrestM. Detamore
- 2014
Engineering, Medicine
Cellular reprogramming
It is shown that using Y-27632-RI in concert with nucleofection could greatly enhance the utility of differentiating and reprogramming hUCMSCs for tissue engineering applications.
- 15
- PDF
- Qingjun ZhouH. DuanYao WangMingli QuLingling YangLixin Xie
- 2013
Biology, Medicine
Tissue engineering. Part C, Methods
Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 increased the cloning efficiency of rabbit limbal stem/progenitor cells by improving their adherence and ROS scavenging capacity.
- 28
- PDF
...
...
45 References
- Xiangyun LiG. MengR. KrawetzShiying LiuD. Rancourt
- 2008
Medicine, Biology
Stem cells and development
It is concluded that conventional slow freezing with Y-27632 treatment is efficient and convenient for the cryopreservation of hES cells.
- 114
- C. BuenoR. MontesP. Menéndez
- 2010
Biology, Medicine
Stem Cell Reviews and Reports
This study identifies for the first time a detrimental effect of Y-27632 on the expansion and survival of both fresh and cryopreserved CB-derived CD34+ HSPCs, suggesting that Y- 27632 may have a differential impact on distinct lineage/tissue-specific stem cells.
- 48
- PDF
- Mohammad PakzadM. TotonchiA. TaeiA. SeifinejadSeyedeh-Nafiseh HassaniH. Baharvand
- 2009
Biology, Medicine
Stem Cell Reviews and Reports
Results show that addition of ROCKi in the extracellular matrix can increase the plating efficiency of hESCs and hiPSCs during passaging of clusters due not only to an anti-apoptotic effect, but also to an increase in the ECM-cells interaction.
- 93
- Kiichi WatanabeMorio Ueno Y. Sasai
- 2007
Biology, Medicine
Nature Biotechnology
Application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency and facilitates subcloning after gene transfer, and enables SFEB-cultured hES Cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors.
- 2,001
- PDF
- David ClaassenM. DeslerA. Rizzino
- 2009
Medicine, Biology
Molecular reproduction and development
It is demonstrated that Y‐27632, a specific inhibitor of Rho kinase (ROCK) activity, significantly enhances recovery of hES cells from cryopreserved stocks when cultured with or without a growth inactivated feeder layer, and argues that this tool is a useful tool in overcoming obstacles to studies involving the cultivation of both h ES cells and human iPS cells.
- 165
- PDF
- B. Heng
- 2009
Medicine
- 73
- Toshiyuki TakeharaTakeshi Teramura Y. Hosoi
- 2008
Biology, Medicine
Molecular human reproduction
The present result suggests that the chemical was effective for improving the culture system of primate ES cells, but also the similarity between cyES and hES cells regarding the reactions to the chemical, which might be further evidence that cyES cells are superior models for h ES cells.
- 23 [PDF]
- Raquel Martín-IbañezRaquel Martín-Ibañez O. Hovatta
- 2008
Biology, Medicine
Human reproduction
This novel method for the cryopreservation of dissociated hESCs may reduce the time required to amplify frozen stocks, and facilitate not only the storage of large numbers of hESC but also the widespread use of these cells in regenerative medicine.
- 119
- PDF
- Xiangyun LiR. KrawetzShiying LiuG. MengD. Rancourt
- 2009
Medicine, Biology
Human reproduction
A novel method to increase freeze-thaw survival and proliferation rate of single hES cells in serum-free and feeder-free culture conditions and it is believed this method will be ideal for current and future hES cell applications that are targeted towards a therapeutic end-point.
- 172
- PDF
- S. BraamRoy NauwD. Ward-van OostwaardC. MummeryR. Passier
- 2010
Biology, Medicine
Annals of the New York Academy of Sciences
In conclusion, inhibition of the Rho‐associated kinase (ROCK) by Y‐27632 improved survival of dissociated hESC‐derived differentiated cells and facilitated development of applications of hESCs‐derived cardiomyocytes in multiple research areas.
- 35
...
...
Related Papers
Showing 1 through 3 of 0 Related Papers